Montgomery P C, Rockey J H, Majumdar A S, Lemaitre-Coelho I M, Vaerman J P, Ayyildiz A
Invest Ophthalmol Vis Sci. 1984 Mar;25(3):369-73.
Rats were immunized repeatedly with dinitrophenylated type III pneumococcal vaccine by the intravenous (IV), subcutaneous (SC), gastrointestinal (GI), or ocular/topical (OT) routes at biweekly intervals. IgA anti-DNP antibodies were measured in serum, tears, saliva, bronchial, and intestinal washings, obtained 7 days after the third and sixth immunizations, using a solid phase radioimmunoassay. The GI route most effective at eliciting and maintaining IgA antibody responses in tears. The OT group displayed markedly diminished IgA response frequencies and antibody levels in tears following prolonged immunization. These data show that repeated central mucosal (gastrointestinal associated lymphoid tissue) stimulation maintains a local IgA response in tears, while continued topical antigen stimulation does not. Isoelectric focusing was used to probe the spectral complexity of the IgA antibodies of individual animals undergoing GI and OT immunization. The reduction of spectral complexity and the decreased responses following OT immunization appear to reflect a diminution of IgA antibody producing cells in the lacrimal gland. The concomitant changes in spectral components and maintenance of responsiveness of the GI group suggests that central mucosal site stimulation provides the lacrimal compartment with a continuous but variable population of IgA antibody producing cells.
将二硝基苯基化III型肺炎球菌疫苗通过静脉内（IV）、皮下（SC）、胃肠道（GI）或眼/局部（OT）途径，每隔一周给大鼠重复免疫一次。在第三次和第六次免疫后7天，通过固相放射免疫测定法测量血清、眼泪、唾液、支气管和肠道灌洗液中的IgA抗DNP抗体。胃肠道途径在诱导和维持眼泪中的IgA抗体反应方面最为有效。在长期免疫后，OT组眼泪中的IgA反应频率和抗体水平明显降低。这些数据表明，反复的中枢黏膜（胃肠道相关淋巴组织）刺激可维持眼泪中的局部IgA反应，而持续的局部抗原刺激则不能。等电聚焦用于探测接受GI和OT免疫的个体动物的IgA抗体的光谱复杂性。OT免疫后光谱复杂性的降低和反应的减少似乎反映了泪腺中产生IgA抗体的细胞数量减少。GI组光谱成分的伴随变化和反应性的维持表明，中枢黏膜部位刺激为泪腺提供了连续但可变的产生IgA抗体的细胞群体。
