Martin L B, Montgomery P C, Holland T C
Department of Immunology and Microbiology, Wayne State University School of Medicine, Detroit, Michigan 48201.
J Virol. 1992 Sep;66(9):5183-9. doi: 10.1128/JVI.66.9.5183-5189.1992.
Herpes simplex virus type 1 (HSV-1) ocular infection in rats was blocked by treating the eyes with UV-inactivated virions containing glycoprotein D (gD) prior to ocular challenge. In contrast, rats treated with UV-inactivated virions lacking gD were not protected. A soluble, truncated form of HSV-2 gD (gD-2t) also protected against ocular infection. Treatment with gD-2t not only reduced mortality but also restricted progression of pathology and reduced the amount of viral antigen in the cornea. Host antibody or alpha/beta interferon responses to the gD-2t treatment were not detected. These results are similar to those observed in cell culture (D. C. Johnson, R. L. Burke, and T. Gregory, J. Virol. 64:2569-2576, 1990). The in vivo effect of exogenous gD is consistent with blocking of a cell surface gD receptor or with an inhibitory interaction of gD with virions.
在眼部攻击前，用含有糖蛋白D（gD）的紫外线灭活病毒颗粒处理大鼠眼睛，可阻断1型单纯疱疹病毒（HSV-1）的眼部感染。相比之下，用缺乏gD的紫外线灭活病毒颗粒处理的大鼠未得到保护。HSV-2 gD的可溶性截短形式（gD-2t）也可预防眼部感染。用gD-2t处理不仅降低了死亡率，还限制了病理进展，并减少了角膜中病毒抗原的量。未检测到宿主抗体或α/β干扰素对gD-2t处理的反应。这些结果与在细胞培养中观察到的结果相似（D. C. 约翰逊、R. L. 伯克和T. 格雷戈里，《病毒学杂志》64:2569 - 2576, 1990）。外源性gD的体内作用与阻断细胞表面gD受体或gD与病毒颗粒的抑制性相互作用一致。
