Kirkegaard P, Olsen P S, Poulsen S S, Nexø E
Regul Pept. 1983 Dec;7(4):367-72. doi: 10.1016/0167-0115(83)90108-8.
Brunner's glands of the duodenum are innervated by cholinergic and VIP-ergic nerves, and the glands have been shown to contain epidermal growth factor (EGF). In this study the effect of VIP and acetylcholine (Ach) on secretion of EGF from Brunner's glands was investigated in the rat. Intravenous infusion of VIP stimulated the flow rate of duodenal secretion, an effect which was inhibited by atropine. Ach alone did not significantly increase flow rate, and combined infusion of VIP and Ach induced the same flow as VIP alone. Concentration of EGF in duodenal secretion was increased by infusion of Ach, and this effect was potentiated by VIP. Infusion of VIP alone did not influence EGF concentration. EGF output from Brunner's glands was significantly stimulated by i.v. infusion of VIP and of Ach and combined infusion further increased EGF output. The study has demonstrated exocrine secretion of EGF from Brunner's glands, and it is suggested that stimulation is mediated by interaction of neuronal VIP and Ach.
十二指肠的Brunner腺由胆碱能神经和血管活性肠肽（VIP）能神经支配，并且已证明这些腺体含有表皮生长因子（EGF）。在本研究中，研究了VIP和乙酰胆碱（Ach）对大鼠Brunner腺分泌EGF的影响。静脉输注VIP可刺激十二指肠分泌的流速，阿托品可抑制该作用。单独使用Ach不会显著增加流速，VIP和Ach联合输注诱导的流速与单独使用VIP相同。输注Ach可增加十二指肠分泌物中EGF的浓度，VIP可增强该作用。单独输注VIP不影响EGF浓度。静脉输注VIP、Ach以及联合输注均显著刺激了Brunner腺的EGF分泌量，联合输注进一步增加了EGF分泌量。该研究证明了Brunner腺可外分泌EGF，提示其分泌受神经元VIP和Ach相互作用的介导。
