Kirkegaard P, Olsen P S, Nexø E, Holst J J, Poulsen S S
Gut. 1984 Nov;25(11):1225-9. doi: 10.1136/gut.25.11.1225.
The effect of VIP and somatostatin on secretion of epidermal growth factor and bicarbonate from Brunner's glands was investigated in the rat. Vasoactive intestinal polypeptide infused in doses of 10 and 100 ng/kg/h significantly increased epidermal growth factor and bicarbonate output, but the concentrations did not change. Somatostatin infused at doses of 1, 10, 100 and 1000 ng/kg/h against a background of VIP 100 ng/kg/h inhibited in dose-dependent fashion the stimulated epidermal growth factor and bicarbonate outputs from rat Brunner's gland pouches. Also basal secretion was inhibited by somatostatin. Infusion of antisomatostatin serum stimulated Brunner's gland secretion. By immunohistochemical studies of rat duodena, it was found that epidermal growth factor, is almost exclusively present in the secretory cells of Brunner's glands. It is concluded that VIP stimulates secretion of epidermal growth factor and bicarbonate from Brunner's glands, an effect which is inhibited by somatostatin. A possible role for somatostatin in the control of Brunner's gland secretion is suggested.
在大鼠中研究了血管活性肠肽（VIP）和生长抑素对十二指肠腺表皮生长因子和碳酸氢盐分泌的影响。以10和100 ng/kg/h的剂量输注血管活性肠肽可显著增加表皮生长因子和碳酸氢盐的分泌量，但浓度未发生变化。在100 ng/kg/h的VIP背景下，分别以1、10、100和1000 ng/kg/h的剂量输注生长抑素，以剂量依赖的方式抑制了大鼠十二指肠腺小袋中受刺激的表皮生长因子和碳酸氢盐的分泌。生长抑素也抑制基础分泌。输注抗生长抑素血清可刺激十二指肠腺分泌。通过对大鼠十二指肠进行免疫组织化学研究发现，表皮生长因子几乎仅存在于十二指肠腺的分泌细胞中。得出的结论是，VIP刺激十二指肠腺分泌表皮生长因子和碳酸氢盐，而生长抑素可抑制这一作用。提示生长抑素在十二指肠腺分泌控制中可能发挥作用。
