Mossalayi M D, Goube de Laforest P, Guilhot F, Lemaire M, Larroque V, Tanzer J
Int J Cell Cloning. 1984 Jan;2(1):34-46. doi: 10.1002/stem.5530020106.
T-cell colonies derived from peripheral blood mononuclear cells plated in agar with phytohemagglutinin (PHA) contain about 5-7% E-, OKT3-, OKT6-, TdT-, SIg- cells phenotypically similar to bone marrow precursors since they are also RFB1+, OKT10+, HLA-DR+, and PNA+. These cells can generate secondary E+, OKT3+ T-cell colonies when replated in the presence of both PHA and a colony-promoting activity distinct from the T-cell growth factor, Interleukin 2. These observations suggest that E-, OKT3- cells detected in primary PHA-induced T-cell colonies belong to a pool of prethymic cells which are able to mature into T-lymphocytes of T4 and T8 type in the absence of the thymic microenvironment.
用植物血凝素（PHA）接种于琼脂中的外周血单个核细胞衍生的T细胞集落含有约5 - 7%的E -、OKT3 -、OKT6 -、TdT -、SIg -细胞，其表型与骨髓前体细胞相似，因为它们也是RFB1 +、OKT10 +、HLA - DR +和PNA +。当在PHA和一种不同于T细胞生长因子白细胞介素2的集落促进活性存在的情况下再次接种时，这些细胞可以产生次级E +、OKT3 + T细胞集落。这些观察结果表明，在原发性PHA诱导的T细胞集落中检测到的E -、OKT3 -细胞属于胸腺前细胞池，它们能够在没有胸腺微环境的情况下成熟为T4和T8型T淋巴细胞。
