Goube de Laforest P, Lasmayous-Riou N, Alcalay D, Tanzer J
Immunology. 1978 Dec;35(6):917-22.
PHA-induced colonies were obtained from peripheral blood mononuclear cells (MC) grown in agar-medium. When the colonies were harvested from mass cultures, pooled as single cell suspensions and plated again in presence of PHA, they failed to generate new colonies unless they were seeded on an underlayer containing uncultured blood MC. Cytogenetic studies indicate that most secondary colonies were derived from primary colonies. Autologous as well as heterologous feeder cells were able to promote the growth of secondary colonies. No granulocyte (G) or macrophage (M) colony formation was observed in secondary cultures. These experiments show that the progenitors of PHA-induced colonies differ from G or M CFCs and that they are still detected in these colonies which contain 82 +/- 12% T-cells. In contrast, colony formation requires the presence of factor(s) provided by cooperating cells (CC) which are no longer detected in primary colonies and this is associated with a depletion in non-T elements from the initial MC population.
从在琼脂培养基中生长的外周血单个核细胞（MC）获得PHA诱导的集落。当从大量培养物中收获集落，合并为单细胞悬液并在PHA存在下再次接种时，除非将它们接种在含有未培养的血液MC的底层上，否则它们无法产生新的集落。细胞遗传学研究表明，大多数次级集落源自初级集落。自体以及异源饲养细胞能够促进次级集落的生长。在次级培养物中未观察到粒细胞（G）或巨噬细胞（M）集落形成。这些实验表明，PHA诱导的集落的祖细胞不同于G或M CFC，并且在这些含有82±12% T细胞的集落中仍可检测到它们。相反，集落形成需要由协作细胞（CC）提供的因子存在，而在初级集落中不再检测到这些因子，这与初始MC群体中非T元素的消耗有关。
