Yamamoto R S, Johnson D L, Masunaka I K, Granger G A
J Biol Response Mod. 1984;3(1):76-87.
The phorbol ester 4 beta-phorbol 12-myristate 13-acetate (PMA) was studied for its ability to induce increased lymphotoxin (LT) production from a number of continuous human B lymphoblastoid cell lines that spontaneously release low levels of LT in vitro. A 5-20-fold increase in LT production was seen when all seven of these cell lines were cocultured with 20 ng/ml PMA for 72 h under serum-free conditions in 0.1% lactalbumin hydrolysate. One cell line, GM3104A, was cloned and repeatedly selected to obtain a high-level LT producer in the presence of PMA. This subline, termed IR 3.4, spontaneously released the alpha-LT molecular weight (MW) class; however, upon PMA stimulation it released high levels of alpha and an unrelated beta-LT MW class form. The increased amounts of LT released in the presence of PMA by IR 3.4 lymphoid cells provides a new method for the production of large amounts of alpha-LT for purification from a single cell source for biochemical and functional studies, and indicates that the type of induction signal may influence the type of LT released.
研究了佛波酯4β-佛波醇12-肉豆蔻酸酯13-乙酸酯（PMA）诱导多种连续人B淋巴母细胞系产生更多淋巴毒素（LT）的能力，这些细胞系在体外自发释放低水平的LT。当所有这七种细胞系在无血清条件下于0.1%水解乳白蛋白中与20 ng/ml PMA共培养72小时时，LT产量增加了5至20倍。克隆了一个细胞系GM3104A，并反复筛选以获得在PMA存在下能高水平产生LT的细胞系。这个亚系称为IR 3.4，自发释放α-LT分子量（MW）类别；然而，在PMA刺激下，它释放高水平的α和一种不相关的β-LT MW类别形式。IR 3.4淋巴细胞在PMA存在下释放的LT量增加，为从单一细胞来源大量生产α-LT用于生化和功能研究的纯化提供了一种新方法，并表明诱导信号的类型可能影响释放的LT类型。
