Asada M, Higuchi M, Kobayashi Y, Osawa T
Cell Immunol. 1983 Apr 1;77(1):150-60. doi: 10.1016/0008-8749(83)90015-1.
Human lymphotoxin (LT)-producing T-cell hybridomas were constructed by fusing concanavalin A-activated human peripheral blood lymphocytes with emetine-actinomycin D-pretreated human acute lymphatic leukemia cells. LT secretion from these hybridomas was considerably enhanced by stimulation with phorbol-12-myristate-13-acetate (PMA) and concanavalin A or PMA alone. A study using cloned hybrid lines revealed that PMA/Con A acted directly on the LT-producing clones. Furthermore, PMA/Con A stimulated A-B9-24, one of the cloned hybridomas, and secreted fourfold larger amounts of LT under serum-free conditions than under serum-containing conditions. However, MIF/MAF and LT-producing cloned hybrid line E10-20 secreted rather decreased amounts of MIF/MAF when stimulated with PMA, while the LT secretion from the same hybridoma was enhanced with PMA.
通过将伴刀豆球蛋白A激活的人外周血淋巴细胞与经吐根碱-放线菌素D预处理的人急性淋巴细胞白血病细胞融合，构建了产生人淋巴毒素（LT）的T细胞杂交瘤。用佛波醇-12-肉豆蔻酸酯-13-乙酸酯（PMA）和伴刀豆球蛋白A或单独使用PMA刺激可显著增强这些杂交瘤的LT分泌。一项使用克隆杂交系的研究表明，PMA/伴刀豆球蛋白A直接作用于产生LT的克隆。此外，PMA/伴刀豆球蛋白A刺激克隆杂交瘤之一A-B9-24，在无血清条件下分泌的LT量比在含血清条件下多四倍。然而，用PMA刺激时，MIF/MAF和产生LT的克隆杂交系E10-20分泌的MIF/MAF量反而减少，而同一杂交瘤的LT分泌则因PMA而增强。
