Keren Z, Berke G
Cell Immunol. 1984 May;85(2):556-62. doi: 10.1016/0008-8749(84)90268-5.
Soluble concanavalin A (Con A) can effectively mediate nonspecific target cell lysis by cytolytic T lymphocytes (LDCC). Because Con A bound to Sepharose beads (Con A-Seph) is also effective, it has been concluded by Z. K. Ballas, W. R. Green, and C. S. Henney. (Cell. Immunol. 59, 411, 1981) that Con A-mediated "activation" of the cytolytic cell to kill in LDCC can occur without intracellular penetration of the lectin. No preincubation of either effector or target cells with Con A-Seph has been performed. Exploiting the previous finding of G. Berke (Immunol. Rev. 72, 5, 1983) that in LDCC Con A exerts its effect(s) strictly by affecting the target rather than by bridging effector and target cells and activating the effector, identical results with Con A-Seph are shown. Preincubation of Con A-Seph with the target but not with the effector cells results in substantial killing. Moreover it is shown that the ability of Con A-Seph to mediate LDCC can be attributed to free Con A dissociating from the beads (about 1%) during the assay. Evidence is presented to indicate that the dissociated Con A, not unlike free Con A, reacts with the target cells, thereby rendering them recognizable by the effector cells. It is concluded that the activity of Con A-Seph may not be taken as evidence for Con A-mediated activation of the cytolytic cell, as suggested by Ballas et al., and that the putative Con A-mediated lymphocyte activation relevant to killing still remains to be demonstrated. Evidence contradicting Con A-mediated activation of the effector and supporting the target cell modification theory has been discussed by G. Berke, V. Hu, E. McVey, and W. R. Clark (J. Immunol. 127, 776, 1981).
可溶性伴刀豆球蛋白A（Con A）可有效介导细胞毒性T淋巴细胞进行非特异性靶细胞裂解（LDCC）。由于结合在琼脂糖珠上的Con A（Con A-Seph）也具有同样的效果，Z. K. 巴拉斯、W. R. 格林和C. S. 亨尼（《细胞免疫学》第59卷，第411页，1981年）据此得出结论，在LDCC中，Con A介导的溶细胞的“激活”作用可以在凝集素不穿透细胞内部的情况下发生。之前没有对效应细胞或靶细胞进行Con A-Seph预孵育。利用G. 伯克（《免疫学评论》第72卷，第5页，1983年）之前的发现，即在LDCC中Con A严格通过影响靶细胞发挥作用，而不是通过连接效应细胞和靶细胞并激活效应细胞，本研究展示了Con A-Seph产生的相同结果。Con A-Seph与靶细胞而非效应细胞进行预孵育会导致大量细胞死亡。此外，研究表明Con A-Seph介导LDCC的能力可归因于在检测过程中从珠子上解离的游离Con A（约1%）。有证据表明，解离的Con A与游离的Con A一样，会与靶细胞发生反应，从而使它们能够被效应细胞识别。研究得出结论，Con A-Seph的活性可能不能像巴拉斯等人所认为的那样，作为Con A介导的溶细胞激活的证据，与杀伤相关的假定的Con A介导的淋巴细胞激活仍有待证实。G. 伯克、V. 胡、E. 麦克维及W. R. 克拉克（《免疫学杂志》第127卷，第776页，1981年）已讨论了与Con A介导的效应细胞激活相矛盾以及支持靶细胞修饰理论的证据。
