Southard J H, Lutz M F, Ametani M S, Belzer F O
Cryobiology. 1984 Feb;21(1):13-9. doi: 10.1016/0011-2240(84)90017-8.
During continuous hypothermic perfusion of dog kidneys there occurs a gradual decrease in ATP from about 1.4 to 0.6 mumol/g wet wt after 5 days of preservation. The loss of ATP can be prevented by including both adenosine (10 mM) and PO4 (25 mM) in the perfusate. Under these conditions kidney cortex ATP levels were more than double control values--3.5 mumol/g wet wt. Both adenosine and PO4 were necessary since omission of one substance resulted in no net synthesis of ATP. Furthermore, these high levels of ATP were obtained only if adequate concentrations of adenosine were maintained during perfusion. Following 3 days of perfusion the adenosine level in the perfusate decreased to about 1 mM and under this condition ATP levels were low. Adenosine levels were maintained in the perfusate by two methods: (1) addition of fresh perfusate or (2) pretreatment of the kidney with the adenosine deaminase inhibitor--deoxycoformycin. The increased levels of ATP appear directly related to the availability of nucleotide precursors and the presence of inhibitors of the enzymes involved in the catabolism of nucleotides and nucleosides (PO4 and deoxycoformycin). Mitochondrial activity was similar in kidneys with high or low ATP levels following 5 days of preservation.
在对狗肾进行持续低温灌注期间，保存5天后，ATP会从约1.4微摩尔/克湿重逐渐降至0.6微摩尔/克湿重。通过在灌注液中加入腺苷（10毫摩尔）和磷酸根（25毫摩尔），可以防止ATP的损失。在这些条件下，肾皮质的ATP水平是对照值的两倍多——3.5微摩尔/克湿重。腺苷和磷酸根都是必需的，因为缺少其中一种物质都不会导致ATP的净合成。此外，只有在灌注期间维持足够浓度的腺苷，才能获得这些高水平的ATP。灌注3天后，灌注液中的腺苷水平降至约1毫摩尔，在此条件下ATP水平较低。通过两种方法维持灌注液中的腺苷水平：（1）添加新鲜灌注液；（2）用腺苷脱氨酶抑制剂——脱氧助间型霉素对肾脏进行预处理。ATP水平的升高似乎与核苷酸前体的可用性以及参与核苷酸和核苷分解代谢的酶的抑制剂（磷酸根和脱氧助间型霉素）的存在直接相关。保存5天后，ATP水平高或低的肾脏中的线粒体活性相似。
