Postlethwaite A E, Smith G N, Mainardi C L, Seyer J M, Kang A H
J Immunol. 1984 May;132(5):2470-7.
Increased collagen deposition is a common feature of granulomatous and nongranulomatous inflammation associated with certain types of cell-mediated immune reactions in vivo. In the present study, we found that normal human peripheral blood mononuclear leukocytes cultured in vitro and stimulated by antigens or T cell mitogens release a 100 to 170 K m.w., heat-labile, trypsin-sensitive protein that stimulates dermal fibroblasts to produce increased quantities of type I and III collagens. Our data suggest that this collagen production protein is of T lymphocyte origin and that it preferentially stimulates production of collagen. We also observed that human mononuclear leukocytes release a different effector molecule with an m.w. of 55 K that inhibits fibroblast collagen production. Mononuclear leukocytes in culture are capable of releasing both the stimulator and the inhibitor of collagen production. The relative amounts of each of these factors elaborated by mononuclear leukocytes in culture appear to be influenced by several variables, such as cell density, type of stimulant used, and the duration of the culture period. These observations suggest that collagen production by fibroblasts in close proximity to sites in vivo where cell-mediated immune reactions are occurring might be regulated by both of these effector molecules.
胶原蛋白沉积增加是体内某些类型细胞介导的免疫反应相关的肉芽肿性和非肉芽肿性炎症的共同特征。在本研究中，我们发现体外培养并用抗原或T细胞促有丝分裂原刺激的正常人外周血单个核白细胞释放一种分子量为100至170K、热不稳定、对胰蛋白酶敏感的蛋白质，该蛋白质刺激真皮成纤维细胞产生更多量的I型和III型胶原蛋白。我们的数据表明，这种胶原蛋白生成蛋白起源于T淋巴细胞，并且它优先刺激胶原蛋白的产生。我们还观察到人类单核白细胞释放一种分子量为55K的不同效应分子，该分子抑制成纤维细胞胶原蛋白的产生。培养中的单核白细胞能够释放胶原蛋白产生的刺激物和抑制剂。培养中的单核白细胞产生的这些因子中每种的相对量似乎受几个变量影响，如细胞密度、所用刺激物的类型以及培养期的持续时间。这些观察结果表明，靠近体内发生细胞介导免疫反应部位的成纤维细胞的胶原蛋白产生可能受这两种效应分子的调节。
