Farber C M, Liebes L F, Kanganis D N, Silber R
J Immunol. 1984 May;132(5):2543-6.
Lymphocytes from patients with chronic lymphocytic leukemia (CLL) and from normal subjects were incubated with a glucose-glucose oxidase hydrogen peroxide (H2O2) generating system to study the effect of oxidant stress on these cells. Within 4 hr, 90% of normal but only 21% of CLL lymphocytes remained viable. When normal and CLL preparations enriched in B or T cells were exposed to H2O2, B lymphocytes from both groups were highly susceptible to oxidative damage while T lymphocytes were relatively resistant. The H2O2 scavenger catalase prevented the cytotoxicity. The present work identifies the human B lymphocyte as a cell that should be a suitable target for selective killing by H2O2-generating systems.
将慢性淋巴细胞白血病（CLL）患者和正常受试者的淋巴细胞与葡萄糖-葡萄糖氧化酶过氧化氢（H2O2）生成系统一起孵育，以研究氧化应激对这些细胞的影响。在4小时内，90%的正常淋巴细胞仍保持活力，而CLL淋巴细胞只有21%保持活力。当富含B细胞或T细胞的正常和CLL细胞制剂暴露于H2O2时，两组的B淋巴细胞都极易受到氧化损伤，而T淋巴细胞则相对具有抗性。H2O2清除剂过氧化氢酶可防止细胞毒性。本研究确定人类B淋巴细胞是一种适合被H2O2生成系统选择性杀伤的细胞。
