Daemen A J, Buurman W A, vd Linden C J, Groenewegen G, Kootstra G
Vet Immunol Immunopathol. 1984 Jan;5(3):247-58. doi: 10.1016/0165-2427(84)90038-2.
The culture requirements for the production of canine I1-2 are reported. Several parameters have been tested, such as concentration of lectin, length of culture period and presence of the additives serum, polyethylene glycol (PEG) and phorbol myristic acetate (PMA). Optimal results have been obtained by stimulation of peripheral blood leukocytes with the lectin PHA (8 micrograms/ml) for 48 h. Techniques for the production of I1-2 containing supernatant free of PHA have been evaluated. Gel filtration chromatography of culture supernatant revealed that canine I1-2 has a molecular weight (m.w.) of approximately 30,000 daltons, similar to the m.w. of I1-2 produced by murine T cells.
报道了犬白细胞介素-2（I1-2）生产的培养条件。已经测试了几个参数，如凝集素浓度、培养周期长度以及添加剂血清、聚乙二醇（PEG）和佛波酯（PMA）的存在情况。用植物血凝素PHA（8微克/毫升）刺激外周血白细胞48小时可获得最佳结果。已评估了不含PHA的含I1-2上清液的生产技术。培养上清液的凝胶过滤色谱显示，犬I1-2的分子量（m.w.）约为30,000道尔顿，与小鼠T细胞产生的I1-2的分子量相似。
