Murakawa Y, Sakane T
Department of Internal Medicine, Shimane Medical University, Japan.
Arthritis Rheum. 1988 Jul;31(7):826-33. doi: 10.1002/art.1780310702.
In systemic lupus erythematosus (SLE) patients, the production of interleukin-2 (IL-2) by blood T lymphocytes in response to stimulation with phytohemagglutinin (PHA) either alone or with phorbol myristate acetate (PMA) or ionomycin, a Ca2+ ionophore, was examined. Deficiency in PHA-stimulated IL-2 production by cells from SLE patients was repaired by the addition of PMA, but not ionomycin. PMA alone did not stimulate IL-2 production but, in concert with PHA, induced IL-2 synthesis. Moreover, PMA was effective in the repair of the deficiency of PHA-induced IL-2 production by both T4+ and T8+ subsets. Thus, for effective IL-2 production, SLE T cells required signals either distinct from or in addition to those supplied by PHA.
在系统性红斑狼疮（SLE）患者中，研究了血液T淋巴细胞在单独用植物血凝素（PHA）刺激，或与佛波酯肉豆蔻酸酯（PMA）或离子载体A23187（一种Ca2+离子载体）共同刺激时白细胞介素-2（IL-2）的产生情况。添加PMA可修复SLE患者细胞PHA刺激的IL-2产生缺陷，但添加离子载体A23187则不能。单独的PMA不刺激IL-2产生，但与PHA协同作用可诱导IL-2合成。此外，PMA对修复T4+和T8+亚群PHA诱导的IL-2产生缺陷均有效。因此，对于有效的IL-2产生，SLE T细胞需要不同于PHA提供的信号或除PHA提供的信号之外的其他信号。
