Holmberg D, Forsgren S, Ivars F, Coutinho A
Eur J Immunol. 1984 May;14(5):435-41. doi: 10.1002/eji.1830140510.
Attempting to obtain a representative sample of the "natural antibody" repertoire in the developing immune system, we have derived IgM-secreting hybridomas from 4 normal untreated BALB/c mice of the same litter on day 6 after birth. Partially purified IgM preparations obtained in the supernatants of 70 such clones were each screened in binding assays for reactivity with a panel of 9 IgM antibodies, randomly selected from the same collection. Five of these 9 IgM antibodies were found to react with a considerable number of other IgM in the collection, while the other 4 showed only sporadic reactivity. On the other hand, more than half of the 70 antibodies were found to bind specifically to at least one of these five. With a few exceptions, these reactions showed quantitative levels ranging from 5 to 20% of those observed between either of the two interacting IgM and monoclonal rat anti-mu antibodies. The selectivity of these reactions indicated V-region specificity, which was confirmed by analyzing in some detail the reaction between 2 IgM antibodies isolated from the same mouse.
为了在发育中的免疫系统中获取“天然抗体”库的代表性样本，我们在出生后第6天从同一窝的4只未经处理的正常BALB/c小鼠中获得了分泌IgM的杂交瘤。从70个这样的克隆的上清液中获得的部分纯化的IgM制剂，在结合试验中分别与从同一批中随机选择的一组9种IgM抗体进行反应筛选。发现这9种IgM抗体中的5种与该批中的大量其他IgM发生反应，而另外4种仅表现出零星反应。另一方面，发现70种抗体中的一半以上特异性结合这5种中的至少一种。除了少数例外，这些反应的定量水平在两种相互作用的IgM与单克隆大鼠抗μ抗体之间观察到的反应的5%至20%之间。这些反应的选择性表明了V区特异性，通过对从同一只小鼠分离的2种IgM抗体之间的反应进行详细分析得到了证实。
