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针对BALB/c IgM的单克隆抗独特型抗体。重组菌株BALB-Igh-Va/Igh-Cb中V-C交叉的特异性和位点分析。

Monoclonal anti-allotype antibody towards BALB/c IgM. Analysis of specificity and site of a V-C crossover in recombinant strain BALB-Igh-Va/Igh-Cb.

作者信息

Schüppel R, Wilke J, Weiler E

出版信息

Eur J Immunol. 1987 May;17(5):739-41. doi: 10.1002/eji.1830170527.

DOI:10.1002/eji.1830170527
PMID:3582489
Abstract

Polyclonal IgM obtained by fractionation of a BALB/c serum pool was used as the immunogen for C57BL/6 mice. Draining lymph nodes from selected animals donated cells for fusion with myeloma Sp-2/0. Fifteen hybridomas were productive, and one (RS-3.1) was cloned and the affinity-purified product analyzed for its reactivity pattern by MOPC 104E-enzyme-linked immunosorbent assay inhibition. Among five BALB/c myelomas only TEPC 183 (IgM) was active, not those belonging to other Ig classes. Among normal sera from 8 mouse strains only those of BALB/c, DBA/2J and CBA/J showed inhibition. The recombinant strain BALB-Igh-Va/Igh-Cb did not react, which shows that its C mu stems from parental strain C57BL/6, and that therefore the recombination event had occurred 5' of this gene.

摘要

通过对BALB/c血清库进行分级分离获得的多克隆IgM被用作C57BL/6小鼠的免疫原。从选定动物的引流淋巴结中获取细胞，用于与骨髓瘤Sp-2/0融合。15个杂交瘤具有生产能力，其中一个（RS-3.1）被克隆，并通过MOPC 104E酶联免疫吸附测定抑制法对亲和纯化产物的反应模式进行分析。在5种BALB/c骨髓瘤中，只有TEPC 183（IgM）具有活性，其他Ig类别的骨髓瘤则无活性。在8种小鼠品系的正常血清中，只有BALB/c、DBA/2J和CBA/J的血清表现出抑制作用。重组品系BALB-Igh-Va/Igh-Cb没有反应，这表明其Cμ基因来源于亲本品系C57BL/6，因此重组事件发生在该基因的5'端。

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Monoclonal anti-allotype antibody towards BALB/c IgM. Analysis of specificity and site of a V-C crossover in recombinant strain BALB-Igh-Va/Igh-Cb.针对BALB/c IgM的单克隆抗独特型抗体。重组菌株BALB-Igh-Va/Igh-Cb中V-C交叉的特异性和位点分析。
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