Tanokura M, Yamada K
FEBS Lett. 1984 Jun 11;171(2):165-8. doi: 10.1016/0014-5793(84)80480-9.
The changes in intracellular pH and concentration of inorganic phosphate (Pi) were studied during and after contraction of bullfrog sartorius muscles by using 31P-NMR. The intracellular pH was 7.04 in resting muscles at 4 degrees C and an alkalinization by as much as 0.08 pH unit occurred following 5 s tetanic contraction. During the recovery period after contraction the increased level of intracellular pH returned to the resting level, the time course of which coincided with that of the recovery of the Pi concentration. Based on the changes in the intracellular pH and Pi concentration, the buffering power was estimated to be 18 mM/pH unit.
利用31P-核磁共振技术研究了牛蛙缝匠肌收缩期间及收缩后细胞内pH值和无机磷酸盐（Pi）浓度的变化。在4℃下，静息肌肉的细胞内pH值为7.04，5秒强直收缩后发生了高达0.08个pH单位的碱化。在收缩后的恢复期间，细胞内pH值升高的水平恢复到静息水平，其时间进程与Pi浓度的恢复一致。根据细胞内pH值和Pi浓度的变化，估计缓冲能力为18 mM/pH单位。
