Kohase M, Vilcek J
Jpn J Med Sci Biol. 1978 Feb;31(1):17-26. doi: 10.7883/yoken1952.31.17.
Interferon production stimulated with Polyinosinate-Polycytidylate [Poly (I). Poly (C)] in cultures of human FS-4 cells was enhanced ('superinduced') by the irradiation of cells with UV at the time of induction. UV showed no additional enhancing action on interferon production in cultures already superinduced by the sequential treatment with cycloheximide and actinomycin D; UV doses above 1,000 erg/mm2 inhibited interferon synthesis. In UV-irradiated cells interferon production remained sensitive to inhibition by high concentrations of actinomycin D for at least 3 hr after exposure to Poly (I). Poly (C). Irradiation of induced cells at 4, 5 or 6 hr after stimulation with Poly (I). Poly (C) prevented the rapid decline (shutoff) of interferon synthesis seen in control cultures. All these results support the conclusion that the action of UV protects the interferon mRNA from inactivation. This effect, and the fact that interferon mRNA synthesis can occur after the irradiation of cells with superinducing doses of UV, form the basis of the enhancement of interferon production by UV.
在人FS - 4细胞培养物中，用聚肌苷酸 - 聚胞苷酸[聚（I）·聚（C）]刺激产生的干扰素，在诱导时用紫外线照射细胞可使其产量增加（“超诱导”）。紫外线对已经用环己酰亚胺和放线菌素D顺序处理而超诱导的培养物中的干扰素产生没有额外的增强作用；紫外线剂量超过1000尔格/平方毫米会抑制干扰素合成。在用聚（I）·聚（C）处理后，紫外线照射的细胞中干扰素产生对高浓度放线菌素D的抑制作用至少3小时内仍保持敏感。在用聚（I）·聚（C）刺激后4、5或6小时对诱导细胞进行照射，可防止对照培养物中出现的干扰素合成迅速下降（关闭）。所有这些结果都支持这样的结论，即紫外线的作用可保护干扰素mRNA不被灭活。这种效应以及在用超诱导剂量的紫外线照射细胞后仍能发生干扰素mRNA合成这一事实，构成了紫外线增强干扰素产生的基础。
