Atherton K T, Burke D C
J Gen Virol. 1978 Nov;41(2):229-37. doi: 10.1099/0022-1317-41-2-229.
Three different inhibitors of RNA synthesis, actinomycin, alpha-amanitin and camptothecin, and five different inhibitors of protein synthesis were able to superinduce interferon production in human diploid fibroblasts treated with poly(rI).poly(rC). Camptothecin was shown to be a reversible inhibitor of virus induced interferon formation. It also substantially reduced the interferon yield from human diploid fibroblasts which had been superinduced with actinomycin D and cycloheximide. This suggests that the previously reported failure of camptothecin to inhibit interferon production in human diploid cells after induction with poly(rI).poly(rC) is the result of two mutually opposing effects: a marked inhibition of interferon messenger RNA synthesis, but a stimulation of the activity of the interferon messenger RNA that is formed.
三种不同的RNA合成抑制剂，放线菌素、α-鹅膏蕈碱和喜树碱，以及五种不同的蛋白质合成抑制剂，能够在经聚（rI）·聚（rC）处理的人二倍体成纤维细胞中超级诱导干扰素产生。喜树碱被证明是病毒诱导的干扰素形成的可逆抑制剂。它还显著降低了用放线菌素D和环己酰亚胺超级诱导后的人二倍体成纤维细胞的干扰素产量。这表明，先前报道的喜树碱在用聚（rI）·聚（rC）诱导后人二倍体细胞中不能抑制干扰素产生，是两种相互对立作用的结果：对干扰素信使RNA合成的显著抑制，但对所形成的干扰素信使RNA活性的刺激。
