Tasaki I
Jpn J Physiol. 1978;28(1):89-108. doi: 10.2170/jjphysiol.28.89.
Electric responses of extremely small amplitudes (1-30 muV peak-to-peak) repeating with more-or-less definite periodicity could be induced by a variety of chemical stimulants applied to squid giant axons either extracellularly or intracellularly. The chemical stimulants studied include allethrin, aminopyridines, N-bromosuccinimide, dimethylamino-pyridine, glutaraldehyde, osmium tetroxide, parachloromercuribenzoate, rose bengal, scorpion venoms and veratridine. With several mild stimulants, it was possible to evoke periodic miniature responses unaccompanied by a fall in the resting membrane potential. The frequency of miniature responses could be lowered by intracellular injection of TEA (tetraethylammonium). These miniature responses could readily be suppressed by external application of TTX (tetrodotoxin). The miniature responses evoked by 4-aminopyrine were characterized by a large variation in the frequency of responses. Cross-linking of the membrane proteins with a dilute solution of glutaraldehyde produced miniature responses repeating at progressively falling frequencies. Analyses of the processes of production of miniature responses with these and other stimulants have clarified several aspects of the physicochemical properties of the excitable sites of the axon membrane.
向枪乌贼巨大轴突细胞外或细胞内施加多种化学刺激剂，能够诱发幅度极小（峰峰值为1 - 30微伏）且或多或少具有一定周期性重复出现的电反应。所研究的化学刺激剂包括丙烯菊酯、氨基吡啶、N - 溴代琥珀酰亚胺、二甲基氨基吡啶、戊二醛、四氧化锇、对氯汞苯甲酸、孟加拉玫瑰红、蝎毒和藜芦碱。使用几种温和刺激剂时，有可能诱发周期性的微小反应，且静息膜电位不下降。微小反应的频率可通过细胞内注射TEA（四乙铵）降低。这些微小反应很容易被外部施加的TTX（河豚毒素）抑制。4 - 氨基吡啶诱发的微小反应的特点是反应频率变化很大。用稀戊二醛溶液使膜蛋白交联会产生微小反应，其重复频率逐渐降低。对这些及其他刺激剂产生微小反应过程的分析，阐明了轴突膜兴奋部位的一些物理化学性质。
