van Rooijen N, van Nieuwmegen R, Kors N, Eikelenboom P
Anat Rec. 1984 Apr;208(4):579-87. doi: 10.1002/ar.1092080413.
In order to study the distribution pattern of specific antibody-containing cells in the spleen of rabbits during the secondary immune response, rabbits were given two intravenous injections of either free or liposome-associated human serum albumin (HSA) within an interval of 2 months. Demonstration of specific antibody-containing cells was performed by incubation of sections of spleen with HSA-horseradish peroxidase (HRP) conjugates, followed by peroxidase cytochemistry. Specific anti-HSA antibody-containing cells were detected already within 2 days after booster and peak numbers were found 4 days after booster. The bulk of these cells localized in the coaxial lymphocyte sheaths surrounding the terminal arterioles in the spleen. Specific antibody-containing cells were also found in the follicles. Using a double immunoenzyme technique we demonstrated that a majority of the specific antibody-containing cells produced immunoglobulin G(IgG) antibodies. From the results, it is also concluded that, after a priming injection with liposome-associated HSA, liposomes do not further enhance the secondary immune response, when they are also used for the booster injection.
为了研究兔在二次免疫应答期间脾脏中含特异性抗体细胞的分布模式，在两个月的间隔内给兔静脉注射两次游离的或与脂质体结合的人血清白蛋白（HSA）。通过将脾脏切片与HSA-辣根过氧化物酶（HRP）偶联物孵育，然后进行过氧化物酶细胞化学来显示含特异性抗体的细胞。在加强注射后2天内就检测到了特异性抗HSA抗体细胞，加强注射后4天发现细胞数量达到峰值。这些细胞大部分位于脾脏中围绕终末小动脉的同轴淋巴细胞鞘内。在滤泡中也发现了含特异性抗体的细胞。使用双免疫酶技术我们证明，大多数含特异性抗体的细胞产生免疫球蛋白G（IgG）抗体。从结果还可以得出结论，在用与脂质体结合的HSA进行初次注射后，当脂质体也用于加强注射时，它们不会进一步增强二次免疫应答。
