Hrubesová M
Folia Biol (Praha). 1976;22(4):235-45.
Rabbits were injected with one dose of 2-4 mg. isolated nuclear DNA (from spleens of adult donors) at 3-5 days of age and their antibody response to a later administered soluble HSA antigen was followed. A) Rabbits receiving antigen only at 9 days of age, without previous DNA, did not form antibodies even after the second dose of HSA antigen given at 8 weeks of age. Nor were antibodies detected in the serum of rabbits treated before antigen, i.e., at 4-5 days of age, with DNA-N (from spleens of non-immunized rabbits), or DNA-P (from spleens of the pig immunized with a different antigen). Only rabbits treated with DNA-Ia (from spleens of rabbits immunized with soluble HSA antigen) formed antibodies to HSA antigen given at 9 days after birth. Antibodies were detectable in the serum at 21 days after antigen administration and retained increased levels as long as 50 days after the first dose of antigen. B) When HSA antigen was injected at 17-18 days after birth, even the DNA-N (4 mg.) given at 3-5 days after birth increased the antibody response in the recipients. Substantial acceleration and increase in antibody formation was obtained in rabbits treated with DNA-Ib after birth as compared to control rabbits (after antigen only with DNA). On the contrary, DNA-H from spleens of rabbits intensively immunized with HSA in adjuvant, given in a larger dose (4 mg.), suppressed the antibody response of rabbits over 8 weeks of age after the second dose of antigen. DNAs from spleens of immunized donors injected into rabbit offspring, without subsequent HSA antigen administration, did not lead to the appearance of detectable anti-HSA antibodies in the serum of young rabbits under the conditions of our experiments A and B. Long-term effects of DNAs injected in the postnatal period into rabbits on their immune response are discussed.
在3至5日龄时，给兔子注射一剂2 - 4毫克从成年供体脾脏分离的核DNA，然后观察它们对随后给予的可溶性人血清白蛋白（HSA）抗原的抗体反应。A）仅在9日龄时接受抗原而未预先注射DNA的兔子，即使在8周龄时给予第二剂HSA抗原后也未形成抗体。在抗原处理前，即在4至5日龄时用DNA - N（来自未免疫兔子的脾脏）或DNA - P（来自用不同抗原免疫的猪的脾脏）处理的兔子血清中也未检测到抗体。只有用DNA - Ia（来自用可溶性HSA抗原免疫的兔子的脾脏）处理的兔子在出生后9天给予HSA抗原时形成了针对HSA抗原的抗体。在给予抗原后21天血清中可检测到抗体，并且在首次给予抗原后长达50天抗体水平持续升高。B）当在出生后17至18天注射HSA抗原时，即使在出生后3至5天给予的DNA - N（4毫克）也会增强受体中的抗体反应。与对照兔子（仅在抗原处理后给予DNA）相比，出生后用DNA - Ib处理的兔子在抗体形成方面有显著加速和增加。相反，以较大剂量（4毫克）给予来自用HSA佐剂强烈免疫的兔子脾脏的DNA - H，在第二剂抗原后抑制了8周龄以上兔子的抗体反应。在我们的实验A和B的条件下，将免疫供体脾脏的DNA注射到兔后代中，随后不给予HSA抗原，未导致幼兔血清中出现可检测到的抗HSA抗体。讨论了出生后注射到兔子体内的DNA对其免疫反应的长期影响。
