Raul F, von der Decken A
J Nutr. 1983 Oct;113(10):2034-40. doi: 10.1093/jn/113.10.2034.
The effect of sucrose feeding on endogenous intestinal RNA polymerase activities and on chromatin structure was studied in rats. Adult rats were given a 70% sucrose solution for 15 hours following a 48-hour starvation period. Comparison was made with rats starved for 63 hours and with ad libitum nourished animals. Chromatin-bound RNA polymerase I activity was significantly reduced by starvation. Sucrose feeding provoked a significant rise in the activity, but the level found in the nourished rats was not reached. The free poly[d(A-T)]-dependent RNA polymerase I activity of the sucrose-fed rats exceeded that of the starved and the nourished animals. Chromatin-bound RNA polymerase II activity was enhanced most markedly by sucrose feeding. The balance between the chromatin-bound and free enzymes was shifted towards the chromatin-bound state when compared to the starved and nourished rats. Starvation caused a reduction in the size of oligonucleosomes but sucrose feeding restored almost entirely the original pattern obtained in the nourished animals. These results reflect modifications in the structure of chromatin after sucrose feeding. The present report demonstrates that the adaptive processes triggered in the intestine by dietary sucrose are associated with changes in gene expression.
研究了蔗糖喂养对大鼠内源性肠道RNA聚合酶活性及染色质结构的影响。成年大鼠在48小时饥饿期后给予70%蔗糖溶液15小时。将其与饥饿63小时的大鼠以及自由进食的动物进行比较。饥饿显著降低了染色质结合的RNA聚合酶I活性。蔗糖喂养使该活性显著升高，但未达到正常喂养大鼠的水平。蔗糖喂养大鼠的游离聚[d(A-T)]依赖性RNA聚合酶I活性超过了饥饿和正常喂养的动物。蔗糖喂养最显著地增强了染色质结合的RNA聚合酶II活性。与饥饿和正常喂养的大鼠相比，染色质结合酶和游离酶之间的平衡向染色质结合状态转变。饥饿导致寡核小体大小减小，但蔗糖喂养几乎完全恢复了正常喂养动物的原始模式。这些结果反映了蔗糖喂养后染色质结构的改变。本报告表明，饮食蔗糖在肠道中引发的适应性过程与基因表达的变化有关。
