人血清和尿液中免疫调节剂erythro-9-(2-羟基-3-壬基)次黄嘌呤的放射免疫测定

Radioimmunoassay of the immunomodulator erythro-9-(2-hydroxy-3-nonyl)-hypoxanthine in human serum and urine.

作者信息

Pfadenhauer E H, Jones C E, Maxwell K W

出版信息

J Pharm Sci. 1983 Aug;72(8):914-7. doi: 10.1002/jps.2600720818.

DOI:10.1002/jps.2600720818

PMID:6620146

Abstract

A radioimmunoassay was developed for the measurement in human serum and urine of erythro-9-(2-hydroxy-3-nonyl)-hypoxanthine. Antisera were produced in rabbits by immunization with an erythro-9-(2-hydroxy-3-nonyl)-hypoxanthine hemisuccinate-bovine serum albumin conjugate. The competitive antigen was erythro-9-(2-hydroxy-3-nonyl)-hypoxanthine labeled with carbon-14 on the purine ring. Cross-reactivities were measured against three metabolites and the naturally occurring purine bases inosine and hypoxanthine. Sensitivity of the method was 1 ng/ml in serum and 10 ng/ml in urine. Precision at clinical levels was +/- 15% in serum at 2 ng/ml and +/- 3% in urine at 200 ng/ml.

摘要

已开发出一种放射免疫分析法，用于测定人血清和尿液中的赤藓红 - 9 -（2 - 羟基 - 3 - 壬基） - 次黄嘌呤。通过用赤藓红 - 9 -（2 - 羟基 - 3 - 壬基） - 次黄嘌呤半琥珀酸 - 牛血清白蛋白偶联物免疫兔子来制备抗血清。竞争性抗原是在嘌呤环上用碳 - 14标记的赤藓红 - 9 -（2 - 羟基 - 3 - 壬基） - 次黄嘌呤。测定了该方法对三种代谢物以及天然存在的嘌呤碱肌苷和次黄嘌呤的交叉反应性。该方法在血清中的灵敏度为1 ng/ml，在尿液中的灵敏度为10 ng/ml。在临床水平上，血清中2 ng/ml时的精密度为±15%，尿液中200 ng/ml时的精密度为±3%。