Yamada K, Shizuri Y, Ishida Y, Shibata S
J Pharm Sci. 1983 Aug;72(8):945-6. doi: 10.1002/jps.2600720827.
As part of a search for substances from marine organisms, which exhibit inotropic effects, the chemical constituents of the marine green alga, Ulva pertusa were investigated. The fractionated extract was tested for inotropic effects on the isolated guinea pig atria. The aqueous layer obtained from the acetone extract of fresh algae was concentrated, and the residue was extracted with methanol. The methanolic extract was fractionated by chromatography using mixtures of aqueous methanol. Elution with 50% aqueous methanol afforded material that had a significant negative inotropic effect. Further purification of this material by high-performance liquid chromatography (HPLC) using methanol-water (2:5) afforded crystalline adenosine, which was shown to be the active substance of U. pertusa, causing a negative inotropic action.
作为寻找具有变力作用的海洋生物物质的一部分，对海洋绿藻孔石莼的化学成分进行了研究。对分级提取物进行了对离体豚鼠心房变力作用的测试。将新鲜藻类丙酮提取物得到的水层浓缩，残渣用甲醇萃取。甲醇提取物用甲醇水溶液混合物通过色谱法进行分级。用50%甲醇水溶液洗脱得到具有显著负性变力作用的物质。通过使用甲醇 - 水（2:5）的高效液相色谱（HPLC）对该物质进一步纯化，得到结晶腺苷，其被证明是孔石莼的活性物质，可引起负性变力作用。
