Naegele R F, Granoff A
Int J Cancer. 1977 Mar 15;19(3):414-8. doi: 10.1002/ijc.2910190320.
Virus-specific antigens were detected in Lucké tumor cells by indirect immunofluorescence using antiserum prepared against Lucké herpesvirus. Intracellular fluorescence, both cytoplasmic and nuclear, was observed only in acetone:methanol-fixed tumor cells that contained herpesvirus detected by electron microscopy. The number of positive cells correlated well with the number of cells containing virus. In contrast, both virus-containing and virus-free cells exhibited membrane fluorescence when viable unfixed tumor cells were tested. A striking reduction in the number of membrane fluorescent cells was observed with an increase in the length of time that tumor cells were in primary culture. No reaction was observed with a variety of normal R. pipiens cells. Absorption of the antiserum with normal frog kidney tissue had no effect on the number of positive cells whereas absorption with virus-free tumor reduced the reaction; absorption with virus-containing tumor eliminated it. These findings provide the first demonstration that the Lucké herpesvirus genome resident in virus-free tumor cells expresses a virus-associated membrane antigen(s).
使用针对勒克疱疹病毒制备的抗血清，通过间接免疫荧光法在勒克肿瘤细胞中检测到病毒特异性抗原。仅在通过电子显微镜检测到含有疱疹病毒的丙酮：甲醇固定的肿瘤细胞中观察到细胞质和细胞核内的细胞内荧光。阳性细胞的数量与含有病毒的细胞数量密切相关。相反，当测试活的未固定肿瘤细胞时，含病毒和不含病毒的细胞均表现出膜荧光。随着肿瘤细胞原代培养时间的延长，观察到膜荧光细胞的数量显著减少。对多种正常北美豹蛙细胞未观察到反应。用正常蛙肾组织吸收抗血清对阳性细胞数量没有影响，而用无病毒肿瘤吸收则降低了反应；用含病毒肿瘤吸收则消除了反应。这些发现首次证明，存在于无病毒肿瘤细胞中的勒克疱疹病毒基因组表达一种病毒相关膜抗原。
