Lyberg T, Amlie E, Kaplun A, Prydz H
Scand J Immunol. 1983 Sep;18(3):235-40. doi: 10.1111/j.1365-3083.1983.tb00862.x.
The procoagulant activities of non-elicited mouse monocytes/macrophages from four anatomical localizations were compared. These cell populations were further examined for their ability to increase their procoagulant activity on exposure to endotoxin or phytohaemagglutinin (PHA). Peritoneal macrophages exhibited the highest basal procoagulant activity. Their activity was further enhanced by stimulation with endotoxin and PHA, but marked strain differences were noted. This procoagulant has been identified as tissue thromboplastin. Isolated adherent spleen cells, peripheral blood monocytes or lung alveolar macrophages had a low basal procoagulant activity that did not increase on exposure to PHA or endotoxin. The identity of the weak procoagulant in these cells is uncertain. The presence of lymphocytes in the macrophage cultures (4:1 ratio) enhanced slightly (1.3 to 1.5-fold) the response of peritoneal macrophages to endotoxin or PHA but did not significantly influence the procoagulant activity of the other macrophage subpopulations under the conditions tested. These results demonstrate heterogeneity among different macrophage subpopulations with regard to cellular procoagulant expression.
比较了来自四个解剖部位的未激发小鼠单核细胞/巨噬细胞的促凝活性。进一步检测了这些细胞群体在暴露于内毒素或植物血凝素（PHA）时增加其促凝活性的能力。腹腔巨噬细胞表现出最高的基础促凝活性。内毒素和PHA刺激可进一步增强其活性，但存在明显的品系差异。这种促凝剂已被鉴定为组织凝血活酶。分离的贴壁脾细胞、外周血单核细胞或肺泡巨噬细胞的基础促凝活性较低，暴露于PHA或内毒素时活性未增加。这些细胞中弱促凝剂的身份尚不确定。巨噬细胞培养物中淋巴细胞的存在（4:1比例）略微增强（1.3至1.5倍）了腹腔巨噬细胞对内毒素或PHA的反应，但在测试条件下对其他巨噬细胞亚群的促凝活性没有显著影响。这些结果表明，不同巨噬细胞亚群在细胞促凝剂表达方面存在异质性。
