Tertov V V, Orekhov A N, Kosykh V A, Repin V S
Biull Eksp Biol Med. 1982 Oct;94(10):83-5.
Incorporation of 3H-acetate into the main classes of lipids in human aorta cells was studied. It was demonstrated that fractions of intimal and medial cell lipids in primary cultures of unaffected vascular areas were different as to the accumulation of the labeled precursor. Intimal cells isolated from lipid streaks and atherosclerotic plaques accumulated the labeled acetate in lipids 2.5-fold more effectively. The greatest changes in the level of 3H-acetate incorporation were registered in phospholipid fractions and cholesterol esters. Medial cells from the media underlying atherosclerotic lesions were shown to have an enhanced ability to include the label into neutral lipids. It is assumed that the changes in intracellular lipid metabolism may account for the increased lipid content in human atherosclerotic aorta.
研究了3H-醋酸盐掺入人主动脉细胞主要脂质类别的情况。结果表明，未受影响血管区域原代培养物中内膜和中膜细胞脂质部分在标记前体的积累方面存在差异。从脂质条纹和动脉粥样硬化斑块中分离出的内膜细胞在脂质中积累标记醋酸盐的效率高出2.5倍。3H-醋酸盐掺入水平的最大变化出现在磷脂部分和胆固醇酯中。动脉粥样硬化病变下方中膜的中膜细胞显示出将标记物掺入中性脂质的能力增强。据推测，细胞内脂质代谢的变化可能是人类动脉粥样硬化主动脉中脂质含量增加的原因。
