Stalder J, Reigel F, Koblet H
Virology. 1983 Sep;129(2):247-54. doi: 10.1016/0042-6822(83)90164-2.
A persistent infection of Semliki Forest virus (SFV) has been established in Aedes albopictus C6/36 cells. Only a small number of cells survived the initial infection with this RNA virus and gave rise to a persistently infected culture which produced continuously small amounts of infectious virus. To investigate whether defective viral RNA was analyzed early and late after infection by blot hybridizations. Several defective viral RNAs were detected with a common sequence corresponding to the 3' end of the viral genome during and after the establishment of the persistent infection. These defective viral RNAs resemble the defective interfering RNAs in vertebrate cells generated during serial undiluted passages of standard SFV. The defective viral RNAs are rarely released from cells as virions. The rapid generation of defective viral RNAs may be important for the establishment of a persistent infection in mosquito cells.
已在白纹伊蚊C6/36细胞中建立了辛德毕斯病毒（SFV）的持续性感染。最初感染这种RNA病毒后，只有少数细胞存活下来，并产生了持续感染的培养物，该培养物持续产生少量感染性病毒。为了研究感染后早期和晚期是否存在缺陷病毒RNA，通过印迹杂交进行了分析。在持续性感染建立期间和之后，检测到几种具有与病毒基因组3'端相对应的共同序列的缺陷病毒RNA。这些缺陷病毒RNA类似于标准SFV连续未稀释传代过程中在脊椎动物细胞中产生的缺陷干扰RNA。缺陷病毒RNA很少以病毒粒子的形式从细胞中释放出来。缺陷病毒RNA的快速产生可能对在蚊子细胞中建立持续性感染很重要。
