Cotmore S F, Sturzenbecker L J, Tattersall P
Virology. 1983 Sep;129(2):333-43. doi: 10.1016/0042-6822(83)90172-1.
In vitro translation of mRNA from cells infected with the autonomous parvovirus MVM yields four major virally coded proteins. Two of these proteins are indistinguishable both antigenically and by peptide map analysis from the viral capsid polypeptides VP-1 and VP-2. The other two proteins, designated NS-1 and NS-2, are not related to the capsid polypeptides but are recognized by sera from animals infected with different autonomous parvovirus serotypes. The NS-1 protein made in vitro comigrates with VP-1 (MW approximately 83,000), while the NS-2 polypeptide has an apparent molecular weight of 24,000. The transcript for the NS-1 polypeptide was mapped to a block of open reading frame located in the major intron of the left-hand transcription unit in the MVM genome.
用自主细小病毒MVM感染细胞后进行mRNA的体外翻译，可产生四种主要的病毒编码蛋白。其中两种蛋白在抗原性和肽图分析方面，与病毒衣壳多肽VP-1和VP-2无法区分。另外两种蛋白，命名为NS-1和NS-2，与衣壳多肽无关，但可被感染不同自主细小病毒血清型的动物血清识别。体外合成的NS-1蛋白与VP-1（分子量约为83,000）迁移率相同，而NS-2多肽的表观分子量为24,000。NS-1多肽的转录本定位到位于MVM基因组左手转录单元主要内含子中的一个开放阅读框区域。
