Tinker D A, Kung M, Brosnan J T, Herzberg G R
Int J Biochem. 1983;15(10):1225-30. doi: 10.1016/0020-711x(83)90211-2.
Computer analysis of the subcellular distribution of phosphoenolpyruvate carboxykinase in adult female (Gallus domesticus) chicken liver indicates that the activity is distributed between the mitochondria and nucleus in the ratio 70: 30%. There was no activity found in the cytosol. Newly hatched chicks and 8-week-old juveniles were found to have a significant soluble hepatic PEPCK activity. The particulate activity of PEPCK in the livers of juveniles raised in continuous light (24L:0D) decreased over the course of a 7-day fast while no such decrease was seen in juveniles raised in a 12-hr daylength photoperiod (12L:12D).
对成年雌性(家鸡)鸡肝脏中磷酸烯醇式丙酮酸羧激酶的亚细胞分布进行计算机分析表明,该酶活性在线粒体和细胞核之间的分布比例为70:30%。在细胞质中未发现活性。研究发现,刚孵化的雏鸡和8周龄的幼鸡肝脏中有显著的可溶性磷酸烯醇式丙酮酸羧激酶活性。在持续光照(24小时光照:0小时黑暗)条件下饲养的幼鸡肝脏中,磷酸烯醇式丙酮酸羧激酶的颗粒活性在7天禁食过程中下降,而在12小时光照周期(12小时光照:12小时黑暗)条件下饲养的幼鸡中未观察到这种下降。