Yamada M, Matsuda H, Nii S
Gan. 1983 Aug;74(4):502-8.
From MDCC-MSB1, the original Marek's disease virus-associated lymphoblastoid cell line, two sublines named MDCC-MSB1-33C and MDCC-MSB1-41C were differentiated. The MDCC-MSB1-33C cell line grew well at 33 degrees, but grew poorly and finally stopped growing at 41 degrees. On the other hand, the MDCC-MSB1-41C cell line grew actively at 41 degrees, but at 33 degrees, continuous cultivation was difficult to achieve. MDCC-MSB1-33C grew suspended in the liquid medium, as did the original producer MDCC-MSB1 cell line, while MDCC-MSB1-41C grew attached to the substrate. Moreover, in soft agar, MDCC-MSB1-33C formed loose colonies, but MDCC-MSB1-41C formed packed colonies. Neither Marek's disease viral antigens nor herpes-type particles could be detected in either subline, although a Marek's disease tumor-associated surface antigen was found on the surface of both.
从原始的马立克氏病病毒相关淋巴母细胞系MDCC - MSB1分化出了两个亚系，分别命名为MDCC - MSB1 - 33C和MDCC - MSB1 - 41C。MDCC - MSB1 - 33C细胞系在33摄氏度时生长良好，但在41摄氏度时生长不佳并最终停止生长。另一方面，MDCC - MSB1 - 41C细胞系在41摄氏度时生长活跃，但在33摄氏度时难以实现连续培养。MDCC - MSB1 - 33C像原始的产生细胞系MDCC - MSB1一样悬浮在液体培养基中生长，而MDCC - MSB1 - 41C则贴壁生长。此外，在软琼脂中，MDCC - MSB1 - 33C形成松散的菌落，而MDCC - MSB1 - 41C形成紧密的菌落。尽管在两个亚系的表面都发现了马立克氏病肿瘤相关表面抗原，但在这两个亚系中均未检测到马立克氏病病毒抗原或疱疹型颗粒。
