Higashihara T, Mikami T, Kodama H, Izawa H, Tamura H
J Natl Cancer Inst. 1986 Jun;76(6):1085-94.
A monoclonal antibody, 2C12, produced against MDCC-MSB1 cells, reacted with several cell lines derived from Marek's disease (MD), including MDCC-MSB1 cells, normal chicken thrombocytes, and MD tumor cells. It did not react with cell lines derived from avian leukosis or reticuloendotheliosis, cells from normal chicken thymus and bursa, chicken kidney cells and quail fibroblast cultures inoculated with MD virus, or with erythrocytes from 1-day-old and adult chickens, cow, sheep, and horse. Anti-thrombocyte serum prepared in a rabbit reacted with thrombocytes and MDCC-MSB1 cells. The specificities of antibody 2C12 and anti-thrombocyte serum against MDCC-MSB1 cells and thrombocytes were confirmed by cross-absorption, blocking, and double-membrane fluorescent antibody tests. The existence of a polypeptide with an apparent molecular weight of 103,000, common to both MDCC-MSB1 cells and thrombocytes, was demonstrated by Western blotting analyses.
一种针对MDCC-MSB1细胞产生的单克隆抗体2C12，能与多种源自马立克氏病（MD）的细胞系发生反应，包括MDCC-MSB1细胞、正常鸡血小板以及MD肿瘤细胞。它不与源自禽白血病或网状内皮组织增殖病的细胞系、正常鸡胸腺和法氏囊的细胞、鸡肾细胞以及接种了MD病毒的鹌鹑成纤维细胞培养物发生反应，也不与1日龄和成年鸡、牛、羊及马的红细胞发生反应。在兔体内制备的抗血小板血清能与血小板和MDCC-MSB1细胞发生反应。通过交叉吸收、阻断和双膜荧光抗体试验证实了抗体2C12和抗血小板血清对MDCC-MSB1细胞及血小板的特异性。通过蛋白质印迹分析证明了MDCC-MSB1细胞和血小板中均存在一种表观分子量为103,000的多肽。
