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核糖体结合位点上游序列对噬菌体MS2外壳蛋白克隆基因的蛋白质产量的影响。

Effect of the sequences upstream from the ribosome-binding site on the yield of protein from the cloned gene for phage MS2 coat protein.

作者信息

Kastelein R A, Berkhout B, Overbeek G P, van Duin J

出版信息

Gene. 1983 Sep;23(3):245-54. doi: 10.1016/0378-1119(83)90015-x.

DOI:10.1016/0378-1119(83)90015-x
PMID:6628992
Abstract

The translational efficiency of the coat protein gene of phage MS2 has been examined in vivo with respect to neighbouring sequences. The cloned MS2 DNA has been gradually shortened starting at the 5' or 3' terminus, and its effect on coat protein synthesis monitored. Removal of the 3'-terminal sequences had little influence. In contrast, the gradual removal of the 5'-terminal region profoundly reduces translation. Long before the ribosomal binding site (RBS) of the coat protein (CP) gene is reached, the yield of CP has dropped by one order of magnitude. Functional half-lives of the various messengers were found not to be significantly different. Available evidence indicates that the secondary structure of the RBS in native and shortened MS2 RNA is identical. We infer that important determinants for ribosome recognition lie 5' to the RBS region of the MS2 RNA coat gene.

摘要

已在体内研究了噬菌体MS2外壳蛋白基因相对于相邻序列的翻译效率。从5'或3'末端开始逐步缩短克隆的MS2 DNA，并监测其对外壳蛋白合成的影响。去除3'末端序列影响不大。相反，逐步去除5'末端区域会显著降低翻译。早在到达外壳蛋白（CP）基因的核糖体结合位点（RBS）之前，CP的产量就已下降了一个数量级。发现各种信使RNA的功能半衰期没有显著差异。现有证据表明，天然和缩短的MS2 RNA中RBS的二级结构是相同的。我们推断，核糖体识别的重要决定因素位于MS2 RNA外壳基因RBS区域的5'端。

相似文献

1
Effect of the sequences upstream from the ribosome-binding site on the yield of protein from the cloned gene for phage MS2 coat protein.核糖体结合位点上游序列对噬菌体MS2外壳蛋白克隆基因的蛋白质产量的影响。
Gene. 1983 Sep;23(3):245-54. doi: 10.1016/0378-1119(83)90015-x.
2
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J Nanobiotechnology. 2019 May 13;17(1):61. doi: 10.1186/s12951-019-0497-8.
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Unstructured 5'-tails act through ribosome standby to override inhibitory structure at ribosome binding sites.
非结构化 5'-尾部通过核糖体备用来克服核糖体结合位点处的抑制结构。
Nucleic Acids Res. 2018 May 4;46(8):4188-4199. doi: 10.1093/nar/gky073.
4
Direct genetic selection of two classes of R17/MS2 coat proteins with altered capsid assembly properties and expanded RNA-binding activities.对两类具有改变的衣壳组装特性和扩展的RNA结合活性的R17/MS2外壳蛋白进行直接基因选择。
Nucleic Acids Res. 1997 Apr 15;25(8):1649-57. doi: 10.1093/nar/25.8.1649.
5
An unstructured mRNA region and a 5' hairpin represent important elements of the E. coli translation initiation signal determined by using the bacteriophage T7 gene 1 translation start site.一个非结构化的mRNA区域和一个5'发夹结构代表了通过使用噬菌体T7基因1翻译起始位点确定的大肠杆菌翻译起始信号的重要元件。
Nucleic Acids Res. 1993 Dec 11;21(24):5705-11. doi: 10.1093/nar/21.24.5705.
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Mutagenesis of the three bases preceding the start codon of the beta-galactosidase mRNA and its effect on translation in Escherichia coli.β-半乳糖苷酶mRNA起始密码子前三个碱基的诱变及其对大肠杆菌翻译的影响。
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The influence of mRNA primary and secondary structure on human IFN-gamma gene expression in E. coli.mRNA一级和二级结构对人γ-干扰素基因在大肠杆菌中表达的影响。
Nucleic Acids Res. 1984 Oct 25;12(20):7663-75. doi: 10.1093/nar/12.20.7663.
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