Heng E L, Klein D, Debry G
Int J Vitam Nutr Res. 1983;53(3):280-6.
The authors describe an analytical method for the determination of plasma vitamin E by high performance liquid chromatography. 100 microliters of plasma are added to en ethanol-hexane mixture in presence of ascorbic acid. The hexan phase is concentrated and 50 microliters are injected directly into the chromatography in a silica gel column. The detector is set at 280 nm. The very low quantity of plasma required for the determination makes this method very useful in neonatal medicine.
作者描述了一种用高效液相色谱法测定血浆维生素E的分析方法。在抗坏血酸存在的情况下,将100微升血浆加入到乙醇 - 己烷混合物中。将己烷相浓缩,取50微升直接注入硅胶柱色谱仪中。检测器设定在280纳米。该测定所需的血浆量极少,使得这种方法在新生儿医学中非常有用。