高效液相色谱-光电二极管阵列检测法同时测定人血浆中的维生素C、维生素E和β-胡萝卜素
Simultaneous determination of vitamins C, E and beta-carotene in human plasma by high-performance liquid chromatography with photodiode-array detection.
作者信息
Zhao Bin, Tham Su-Yin, Lu Jia, Lai Mui Hoon, Lee Lionel K H, Moochhala Shabbir M
机构信息
Defence Medical and Environmental Research Institute, DSO National Laboratories, (DMERI @ DSO), Singapore, Republic of Singapore.
出版信息
J Pharm Pharm Sci. 2004 Jun 30;7(2):200-4.
PURPOSE
To develop a high-performance liquid chromatography (HPLC) method with photodiode-array ultraviolet detection for the simultaneous determination of vitamin C, vitamin E and beta-carotene.
METHODS
Following liquid-phase extraction from the human plasma samples, these three vitamins were successfully separated on the LiChrospher 100 RP-18 column (125 x 4 mm I.D.; particle size, 5 microm) at a flow-rate of 1.2 ml/min, with a mobile phase of methanol-acetonitrile-tetrahydrofuran (75: 20: 5, v/v/v).
RESULTS
The limits of quantitation were 100, 0.25 and 0.25 microg/ml for vitamin C, vitamin E and beta-carotene respectively. The method is linear over the studied range of 0.25 to 5 g/ml for vitamin E and beta-carotene and 100 to 5000 microg/ml for vitamin C. The extraction recoveries were greater than 83% for these three vitamins. The within day and between-day precision of the analysis did not exceed 15.3 and 16.2%, respectively.
CONCLUSION
A suitable method to determine the concentration of vitamin C, vitamin E and beta-carotene following oral administration of antioxidant supplement capsules to a healthy Chinese volunteer.
目的
建立一种采用光电二极管阵列紫外检测的高效液相色谱（HPLC）方法，用于同时测定维生素C、维生素E和β-胡萝卜素。
方法
从人体血浆样品中进行液相萃取后，这三种维生素在LiChrospher 100 RP - 18柱（内径125×4 mm；粒径5微米）上以1.2 ml/min的流速成功分离，流动相为甲醇 - 乙腈 - 四氢呋喃（75:20:5，v/v/v）。
结果
维生素C、维生素E和β-胡萝卜素的定量限分别为100、0.25和0.25 μg/ml。该方法在维生素E和β-胡萝卜素0.25至5 μg/ml以及维生素C 100至5000 μg/ml的研究范围内呈线性。这三种维生素的萃取回收率均大于83%。分析的日内和日间精密度分别不超过15.3%和16.2%。
结论
一种适用于测定健康中国志愿者口服抗氧化补充剂胶囊后维生素C、维生素E和β-胡萝卜素浓度的方法。
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