马里图巴病毒（布尼亚病毒科）的结构蛋白

Structural proteins of Marituba virus (Bunyaviridae).

作者信息

Frugulhetti I C, Soares M C, Rebello M A

出版信息

Intervirology. 1983;20(2-3):143-8. doi: 10.1159/000149383.

Abstract

Marituba virus was purified by rate-zonal sedimentation and equilibrium density centrifugation in sucrose gradients. The buoyant density of virus particles was 1.19 g/ml. Purified virus was dissociated and its proteins were analyzed by SDS-PAGE. Four virus polypeptides were identified and designated L, G1, G2 and N. Their average molecular weights were 190 x 10(3) [L, range (180-200) x 10(3)], 120 x 10(3) [G1, range (118-122) x 10(3)], 26 x 10(3) [G2, range (24-28) x 10(3)], and 20 x 10(3) [N, range (19-21) x 10(3)]. Polypeptides N and L are, respectively, the major and the minor viral components. G1 and G2 are glycopeptides as demonstrated by the preferential incorporation of labeled 3H-glucosamine.

摘要

马瑞图巴病毒通过蔗糖梯度中的速率区带沉降和平衡密度离心进行纯化。病毒颗粒的浮力密度为1.19克/毫升。纯化的病毒被解离，其蛋白质通过SDS-PAGE进行分析。鉴定出四种病毒多肽，并命名为L、G1、G2和N。它们的平均分子量分别为190×10³[L，范围(180 - 200)×10³]、120×10³[G1，范围(118 - 122)×10³]、26×10³[G2，范围(24 - 28)×10³]和20×10³[N，范围(19 - 21)×10³]。多肽N和L分别是主要和次要的病毒成分。如标记的³H-葡糖胺的优先掺入所示，G1和G2是糖肽。