Danielsen E M, Cowell G M
J Biochem Biophys Methods. 1983 Aug;8(1):41-7. doi: 10.1016/0165-022x(83)90019-2.
A small-scale version of line immunoelectrophoresis in combination with immunoprecipitate excision is described as a rapid and convenient technique to purify proteins on a micro scale in biogenesis studies. In the purification and to result in a higher state of purity than an isolation procedure using protein A-Sepharose. Since the method furthermore allows a simultaneous purification of several different protein antigens from the same sample, it may be of interest as an alternative method to other procedures in the purification of proteins on a micro scale.
本文描述了一种结合免疫沉淀切除的小规模线免疫电泳技术，该技术可在生物发生研究中用于微量蛋白质的快速纯化。在纯化过程中，与使用蛋白A-琼脂糖的分离方法相比，该技术可获得更高的纯度。此外，由于该方法允许从同一样品中同时纯化几种不同的蛋白质抗原，因此作为微量蛋白质纯化中其他方法的替代方法，可能具有一定的应用价值。
