Yamamoto S, Omura M, Hirata H
Vet Immunol Immunopathol. 1985 Jun;9(2):195-200. doi: 10.1016/0165-2427(85)90019-4.
Isolation of porcine, canine and feline IgG has hitherto been achieved usually by DEAE-ion exchange chromatography and gel filtration. These procedures, however, are rather time-consuming, as they involve purification of IgG. Isolation of IgG by affinity chromatography on a column of protein A-Sepharose was attempted. IgG of all three animal species was very easily isolated with a high yield. In the absence of any other method that allows isolation of IgG of three animal species from a single column, the procedure proposed would be very useful.
迄今为止，猪、犬和猫的免疫球蛋白G（IgG）通常是通过二乙氨基乙基（DEAE）离子交换色谱法和凝胶过滤法分离得到的。然而，这些方法相当耗时，因为它们涉及免疫球蛋白G的纯化。有人尝试在蛋白A-琼脂糖柱上通过亲和色谱法分离免疫球蛋白G。所有这三种动物的免疫球蛋白G都很容易以高产率分离出来。鉴于目前尚无任何其他方法能从单一柱上分离这三种动物的免疫球蛋白G，所提出的方法将非常有用。
