Yoshida H, Alexander J C
Lipids. 1983 Sep;18(9):611-6. doi: 10.1007/BF02534671.
The hydrolysis of thermally oxidized sunflower oil by pancreatic lipase was studied in relation to chemical changes in the acylglycerols. Four classes of compounds (monomers, dimers, trimers and polymers) formed from the acylglycerols were separated from the heated oils by column chromatography on silica gel, and further verified by thin layer chromatography. Each fraction, after analyses for generaly properties, was subjected to a time course study of hydrolysis by pancreatic lipase over a 30-min period. After 70 hr of heating, the amount of hydrolysis for the acylglycerol dimers was only about half that of the monomers, and that for the trimers was, in turn, about one-third that of the monomers. The polymers were the least hydrolyzed and showed no further reaction after 5 min. The reduction in enzymatic hydrolysis of isolated fractions from the thermally oxidized oils indicates structural differences, related to formation of polar compounds and polymerization products. Adverse effects on animals from feeding these materials can be attributed partly to inhibition of hydrolysis resulting in less available energy.
研究了胰脂肪酶对热氧化葵花籽油的水解作用及其与酰基甘油化学变化的关系。通过硅胶柱色谱从加热的油中分离出由酰基甘油形成的四类化合物（单体、二聚体、三聚体和聚合物），并通过薄层色谱进一步验证。对每个馏分进行一般性质分析后，对其进行胰脂肪酶在30分钟内的水解时间进程研究。加热70小时后，酰基甘油二聚体的水解量仅约为单体的一半，三聚体的水解量依次约为单体的三分之一。聚合物的水解程度最低，5分钟后不再发生进一步反应。热氧化油中分离出的馏分酶水解的降低表明与极性化合物和聚合产物形成相关的结构差异。给动物喂食这些物质产生的不良影响部分可归因于水解抑制导致能量利用率降低。
