Henderson R J, Burkow I C, Millar R M
N.E.R.C. Unit of Aquatic Biochemistry, Department of Biological and Molecular Science, University of Stirling, Scotland, United Kingdom.
Lipids. 1993 Apr;28(4):313-9. doi: 10.1007/BF02536316.
Fish oils containing different levels of polymers of triacylglycerols formed during autoxidation were incubated with pancreatic lipase to establish whether these polymers are substrates for lipase hydrolysis. With oils containing low amounts (less than 4%) of triacylglycerol polymers as substrates, both triacylglycerols and polymers of triacylglycerols were almost completely hydrolyzed, and fatty acid monomers and monoacylglycerols were the major lipid products. Under the same incubation conditions, some triacylglycerols remained intact when highly oxidized oils containing 20 or 30% triacylglycerol polymers were the substrate. The fatty acid composition of these residual triacylglycerols was almost identical to that of triacylglycerols present at the start of the assay. When fish oil containing 30% triacylglycerol polymers was incubated with the lipase, the component triacylglycerols and polymers of triacylglycerols were hydrolyzed at similar rates, and fatty acid dimers were detected as a product. It is concluded that the high molecular weight polymers of triacylglycerols present in oxidized fish oils can be hydrolyzed by pancreatic lipase in vitro.
将含有不同水平自氧化过程中形成的三酰甘油聚合物的鱼油与胰脂肪酶一起孵育，以确定这些聚合物是否是脂肪酶水解的底物。以含有低含量（低于4%）三酰甘油聚合物的油为底物时，三酰甘油和三酰甘油聚合物几乎完全水解，脂肪酸单体和单酰甘油是主要的脂质产物。在相同的孵育条件下，当以含有20%或30%三酰甘油聚合物的高度氧化油为底物时，一些三酰甘油保持完整。这些残留三酰甘油的脂肪酸组成与测定开始时存在的三酰甘油几乎相同。当含有30%三酰甘油聚合物的鱼油与脂肪酶一起孵育时，三酰甘油组分和三酰甘油聚合物以相似的速率水解，并检测到脂肪酸二聚体作为产物。得出的结论是，氧化鱼油中存在的三酰甘油高分子量聚合物在体外可被胰脂肪酶水解。
