Persky B, Peters E M, Gehlsen K R, Sorrentino J M, Meyskens F L, Hendrix M J
Scan Electron Microsc. 1983(Pt 2):983-95.
A tumorigenic human cell line was derived from a patient with metastatic melanoma. Cells were treated with adriamycin or actinomycin D in order to assess morphological alterations induced by these anticancer agents. Exposure to 0.01 micrograms/ml adriamycin for one hour caused no observable morphological abnormalities as determined by SEM, while 0.1, 1.0 and 10.0 micrograms/ml concentrations of adriamycin produced surface alterations in the form of blebs, filopodia, microvilli and cell rounding. These alterations may be drug-affected changes of the cell surface or may reflect phases of the cell cycle directed by adriamycin action on the nucleus. Cell morphology appeared normal by SEM for 0.01, 0.1, 1.0 and 10.0 micrograms/ml concentrations of adriamycin when the cells were allowed to recover in drug-free media for 24 hours after initial drug incubation. Concentrations of 0.1, 1.0, and 10.0 micrograms/ml actinomycin D for 24 hours created morphological alterations characterized by cell rounding and long, dendritic-like processes. TEM of colonies treated in soft agar for 24 hours with either 1.0 micrograms/ml adriamycin or 1.0 micrograms/ml actinomycin D showed major morphological effects identified by increased cytoplasmic vacuolization and nuclear disintegration.
一种致瘤性人类细胞系源自一名转移性黑色素瘤患者。为了评估这些抗癌药物诱导的形态学改变，细胞用阿霉素或放线菌素D处理。通过扫描电子显微镜（SEM）测定，暴露于0.01微克/毫升阿霉素1小时未引起可观察到的形态学异常，而0.1、1.0和10.0微克/毫升浓度的阿霉素产生了泡状、丝状伪足、微绒毛和细胞变圆形式的表面改变。这些改变可能是药物对细胞表面的影响，也可能反映了阿霉素作用于细胞核所引导的细胞周期阶段。当初始药物孵育后，让细胞在无药物培养基中恢复24小时时，通过SEM观察，0.01、0.1、1.0和10.0微克/毫升浓度的阿霉素处理后的细胞形态看起来正常。0.1、1.0和10.0微克/毫升浓度的放线菌素D处理24小时产生了以细胞变圆和长的树突状突起为特征的形态学改变。用1.0微克/毫升阿霉素或1.0微克/毫升放线菌素D在软琼脂中处理菌落24小时后的透射电子显微镜（TEM）显示，主要的形态学效应表现为细胞质空泡化增加和核解体。
