Breathnach A S, Robins E J, Bhasin Y, Ethridge L, Nazzaro-Porro M, Passi S, Picardo M
Department of Anatomy, St. Mary's Hospital Medical School, London, U.K.
Histol Histopathol. 1987 Jul;2(3):291-7.
Human and murine (Harding-Passey and Cloudman) melanoma cells were exposed to various concentrations (1 x 10(-3) M-1 x 10(-1) M) of adipic (C6), azelaic (C9), and dodecanedioic (C12) acids for 1-6 hours in tissue culture, and the effects on shape and surface topography were examined by scanning electron microscopy. Effects, i.e., rounding up, concentration of microvilli, blebbing, and prominence of retraction fibrils were time and dose dependent, and for the same concentrations and exposure times, C12 had a greater effect than C9, and both a significantly greater effect than C6. These differential reactions to the three diacids parallel previously reported effects on cell kinetics and viability. The changes could be due to a prime effect on the cell membrane, or they might reflect phases of the cell cycle directed by action of the diacids on the nucleus; this latter seems unlikely. An effect on the cytoskeleton is possibly involved.
将人源和鼠源（哈丁 - 帕西和克劳德曼）黑色素瘤细胞在组织培养中暴露于不同浓度（1×10⁻³ M - 1×10⁻¹ M）的己二酸（C6）、壬二酸（C9）和十二烷二酸（C12）中1至6小时，然后通过扫描电子显微镜检查对细胞形状和表面形貌的影响。这些影响，即细胞变圆、微绒毛聚集、细胞膜起泡以及收缩纤维突出，具有时间和剂量依赖性，并且在相同浓度和暴露时间下，C12的影响大于C9，两者的影响均显著大于C6。这三种二元酸的这些差异反应与先前报道的对细胞动力学和活力的影响相似。这些变化可能是由于对细胞膜的主要作用，或者它们可能反映了二元酸作用于细胞核所引导的细胞周期阶段；后者似乎不太可能。可能涉及对细胞骨架的影响。
