Snyder E L, Root R K, McLeod B, Dalmasso A P
Vox Sang. 1983;45(4):288-93. doi: 10.1111/j.1423-0410.1983.tb01916.x.
Fresh blood, stored blood and granulocyte concentrates were passed through 170-micron and microaggregate blood filters to determine the degree of complement activation that occurs during transfusion of citrated blood products. Complement activation was assessed by measurement of C3 conversion using crossed immunoelectrophoresis and by assessment of C5a using a leukocyte aggregation functional assay. Prefiltration, fresh or stored blood products showed 0-1% C3 activation. Postfiltration, the degree of C3 conversion did not change for fresh blood or granulocyte concentrates. For stored whole blood, the degree of C3 conversion increased slightly to 2-3%. Prefiltration results for all samples showed a low level of C5a which did not change after passage through any filter. Serum incubated with filter material at 37 degrees C showed 2-10% C3 conversion. In contrast, results with citrated plasma showed less than 3% conversion of C3. We conclude that although some filter materials may activate complement in serum, filtration of citrated blood products through microaggregate blood filters induces little complement activation.
将新鲜血液、库存血液和粒细胞浓缩液通过170微米和微聚体血液滤器，以确定在输注枸橼酸盐血液制品过程中发生的补体激活程度。通过交叉免疫电泳测量C3转化以及使用白细胞聚集功能测定评估C5a来评估补体激活。预过滤时，新鲜或库存血液制品显示0-1%的C3激活。过滤后，新鲜血液或粒细胞浓缩液的C3转化程度没有变化。对于库存全血，C3转化程度略有增加至2-3%。所有样品的预过滤结果显示C5a水平较低，通过任何滤器后均未改变。在37摄氏度下与滤器材料孵育的血清显示2-10%的C3转化。相比之下，枸橼酸盐血浆的结果显示C3转化率低于3%。我们得出结论，虽然一些滤器材料可能会激活血清中的补体，但通过微聚体血液滤器过滤枸橼酸盐血液制品只会引起很少的补体激活。
