Young G, Kagawa H, Nagahama Y
Biol Reprod. 1983 Sep;29(2):310-5. doi: 10.1095/biolreprod29.2.310.
Aromatase activity in the isolated granulosa layers of amago salmon (Oncorhynchus rhodurus) ovarian follicles was examined during the course of vitellogenesis and final oocyte maturation and ovulation. Estradiol-17 beta production by isolated granulosa layers incubated with exogenous testosterone increased during the period of vitellogenesis to reach a peak in late vitellogenesis, and then declined rapidly, in association with the ability of the oocyte to mature in response to partially purified salmon gonadotropin (SG-G100). Extremely low levels of estradiol-17 beta were produced by granulosa layers from follicles which had undergone final oocyte maturation in vivo and by post-ovulatory follicles. SG-G100 had no discernible effect on estradiol-17 beta production. These results are discussed in relation to other studies on the endocrine control of steroidogenesis in this species.
在卵黄发生、最终卵母细胞成熟和排卵过程中，对秋鲑（Oncorhynchus rhodurus）卵巢卵泡分离颗粒层中的芳香化酶活性进行了检测。在卵黄发生期，与外源性睾酮一起孵育的分离颗粒层产生的雌二醇-17β增加，在卵黄发生后期达到峰值，然后迅速下降，这与卵母细胞对部分纯化的鲑鱼促性腺激素（SG-G100）作出成熟反应的能力相关。体内经历最终卵母细胞成熟的卵泡颗粒层和排卵后卵泡产生的雌二醇-17β水平极低。SG-G100对雌二醇-17β的产生没有明显影响。结合该物种类固醇生成的内分泌控制的其他研究对这些结果进行了讨论。
