Shu Tingting, Chen Yan, Xiao Kan, Huang Hongtao, Jia Jingyi, Yu Zhaoxi, Jiang Wei, Yang Jing
Chinese Sturgeon Research Institute, China Three Gorges Corporation, Yichang, China.
Hubei Key Laboratory of Three Gorges Project for Conservation of Fishes, Yichang, China.
Front Physiol. 2023 Aug 31;14:1248999. doi: 10.3389/fphys.2023.1248999. eCollection 2023.
Since 2011, ecological operation trials of the Three Gorges Reservoir (TGR) have been continuously conducted to improve the spawning quantity of the four major Chinese carp species below the Gezhouba Dam. In particular, exploring the effects of short-term water velocity stimulation on ovarian development in grass carp () is essential to understand the response of natural reproduction to ecological flows. We performed ovary histology analysis and biochemical assays among individuals with or without stimulation by running water. Although there were no obvious effects on the ovarian development characteristics of grass carp under short-term water velocity stimulation, estradiol, progesterone, follicle-stimulating hormone (FSH), and triiodothyronine (T3) concentrations were elevated. Then, we further explored the ovarian development of grass carp under short-term water velocity stimulation by RNA sequencing of ovarian tissues. In total, 221 and 741 genes were up- or downregulated under short-term water velocity stimulation, respectively, compared to the control group. The majority of differentially expressed genes (DEGs) were enriched in pathways including ABC transporters, cytokine-cytokine receptor interaction, ECM-receptor interaction, and steroid hormone biosynthesis. Important genes including , , , , , , and that are involved in ovarian development were regulated. Our results provide new insights and reveal potential regulatory genes and pathways involved in the ovarian development of grass carp under short-term water velocity stimulation, which may be beneficial when devising further ecological regulation strategies.
自2011年以来，三峡水库（TGR）持续开展生态调度试验，以提高葛洲坝下游中华四大家鱼的产卵量。特别是，探究短期水流速度刺激对草鱼（）卵巢发育的影响对于了解自然繁殖对生态流量的响应至关重要。我们对有或没有流水刺激的个体进行了卵巢组织学分析和生化测定。虽然短期水流速度刺激对草鱼的卵巢发育特征没有明显影响，但雌二醇、孕酮、促卵泡激素（FSH）和三碘甲状腺原氨酸（T3）的浓度有所升高。然后，我们通过对卵巢组织进行RNA测序，进一步探究了短期水流速度刺激下草鱼的卵巢发育情况。与对照组相比，在短期水流速度刺激下，分别有221个和741个基因上调或下调。大多数差异表达基因（DEGs）富集在包括ABC转运蛋白、细胞因子-细胞因子受体相互作用、细胞外基质-受体相互作用和类固醇激素生物合成等通路中。参与卵巢发育的重要基因包括、、、、、和等均受到调控。我们的研究结果提供了新的见解，并揭示了短期水流速度刺激下草鱼卵巢发育中潜在的调控基因和通路，这在制定进一步的生态调控策略时可能会有所帮助。
