[溶液离子强度对神经节苷脂菌丝体大小的影响]

[Effect of the ionic strength of solutions on the size of ganglioside mycelia].

作者信息

Dvorkin V M

出版信息

Biull Eksp Biol Med. 1983 Nov;96(11):44-6.

Abstract

Gangliosides in aqueous media of low ionic strength (2-5 mM NaCl) and in concentrations over the critical ones (10(-5) M) form micellas which do not differ from liposomes as regards the chromatographic behavior on Sepharose 4R, with a molecular weight of greater than or equal to 10(7) dalton. In aqueous media of a higher ionic strength (greater than or equal to 20 mM NaCl), gangliosides form micellas which are eluted during chromatography in far later fractions than liposomes 70-80 nm in diameter, with a molecular weight of (1-5) X 10(5) dalton. It is assumed that the conclusions about ganglioside incorporation into the liposomal membrane made on the basis of their peaks coincidence are correct, provided that ganglioside-containing liposomes are obtained and chromatographed under high ionic strength (greater than or equal to 20 mM NaCl).

摘要

在低离子强度（2 - 5 mM NaCl）的水性介质中且浓度超过临界浓度（10⁻⁵ M）时，神经节苷脂会形成胶束，这些胶束在琼脂糖4R上的色谱行为与脂质体无异，其分子量大于或等于10⁷道尔顿。在较高离子强度（大于或等于20 mM NaCl）的水性介质中，神经节苷脂形成的胶束在色谱分析中比直径为70 - 80 nm、分子量为(1 - 5)×10⁵道尔顿的脂质体在更晚的级分中被洗脱。假定基于神经节苷脂峰的重合得出的关于其掺入脂质体膜的结论是正确的，前提是在高离子强度（大于或等于20 mM NaCl）下获得并进行色谱分析含神经节苷脂的脂质体。