Novikov K N, Dudchenko A M, Ugolev A T, Kuznetsova Z I, Luk'ianova L D
Biull Eksp Biol Med. 1983 Nov;96(11):50-2.
Spontaneous destruction of cytochrome P-450 arising from activation of lipid peroxidation (LPO) occurs during incubation of hepatocytes. LPO activation in hepatocyte suspension by a catalytic system containing Fe2+--ADP plus NADP X H makes the destruction of cytochrome P-450 more rapid. Supplementation of the incubation medium with the antioxidant, 2-ethyl-6-methyl-3-hydroxypyridine (HP-6), inhibits LPO, on the one hand, and stabilizes cytochrome P-450, on the other one. Ionol appeared to be a more effective LPO inhibitor in hepatocytes and, accordingly, a more effective stabilizer of cytochrome P-450 than water-soluble HP-6.
肝细胞孵育过程中会发生因脂质过氧化（LPO）激活而导致的细胞色素P - 450自发破坏。含有Fe2 + - ADP加NADP X H的催化系统可激活肝细胞悬液中的LPO，从而使细胞色素P - 450的破坏更快。在孵育培养基中添加抗氧化剂2 - 乙基 - 6 - 甲基 - 3 - 羟基吡啶（HP - 6），一方面可抑制LPO，另一方面可稳定细胞色素P - 450。在肝细胞中，离子醇似乎是一种比水溶性HP - 6更有效的LPO抑制剂，因此也是细胞色素P - 450更有效的稳定剂。
