Isaev E I, Ergashova M Zh, Saatov T S, Turakulov Ia Kh
Biull Eksp Biol Med. 1983 Nov;96(11):54-6.
Administration of alloxan to rats in a dose of 50-150 mg/kg raises blood sugar level from 200 +/- 14 to 800 +/- +/- 16 mg/100 ml. Under these conditions, the content of total gangliosides in the rat femoral muscle progressively decreases, namely from 137 +/- 9 micrograms sialic acid per g tissue to 63 +/- 4, which is linked with alloxan diabetes severity and hyperglycemia. At the same time there was a 3-4-fold inhibition of 14C-glucose oxidation intensity by tissue sections of the skeletal muscles in the presence of a 1.5-2-fold activation of 1-14C-palmitate. Fractionation of glycolipids on silica gel KSK demonstrated a 33% decrease in the content of monosialogangliosides, and a lesser diminution in the content of other fractions. Total muscle gangliosides, added to the incubation medium exogenously, produced a pathogenetic correcting action on disturbed oxidation of 14C-glucose in alloxan diabetes.
给大鼠注射剂量为50 - 150毫克/千克的四氧嘧啶，会使血糖水平从200±14毫克/100毫升升高到800±16毫克/100毫升。在这些条件下，大鼠股四头肌中总神经节苷脂的含量逐渐降低，即从每克组织含137±9微克唾液酸降至63±4微克，这与四氧嘧啶糖尿病的严重程度和高血糖有关。与此同时，在1 - 14C - 棕榈酸酯激活1.5 - 2倍的情况下，骨骼肌组织切片对14C - 葡萄糖的氧化强度受到3 - 4倍的抑制。在硅胶KSK上对糖脂进行分级分离表明，单唾液酸神经节苷脂的含量减少了33%，其他组分的含量减少幅度较小。外源性添加到孵育培养基中的总肌肉神经节苷脂，对四氧嘧啶糖尿病中14C - 葡萄糖氧化紊乱产生了致病纠正作用。
