Fel'dkoren B I, Zil'ber M L, Rogozkin V A
Ukr Biokhim Zh (1978). 1978 May-Jun;50(3):292-5.
A high sensitivity towards cycloheximide is shown for three main protein fractions of albino rat skeletal muscle, i. e. a 50% inhibition of myofibrillar protein synthesis is caused by a dose of 0.1 mg/100 g of body weight after 3.5 h, the rate of inhibition of two other fractions is even higher. A dose of 3.0 mg/100 g of body weight inhibits the synthesis of the sarcoplasmic and myofibrillar proteins by 96% by the end of the 15th minute. Administration of cycloheximide in vivo (0.3 mg/100 g of body weight) inhibits the alpha-amanitin-resistant DNA-dependent RNA-polymerase activity of the nuclei. The relation between the inhibition rate and time of the cycloheximide action is ruled by the first order kinetic. This allows postulating the existence of a fast renewal with a period of half-life of about 110 min (determined graphically) among nuclear proteins involved in the transcription of rRNA. The idea of this protein participation in adaptive shifts of rRNA synthesis in the systematic skeletal muscle function is suggested.
白化大鼠骨骼肌的三种主要蛋白质组分对放线菌酮表现出高敏感性，即3.5小时后，0.1毫克/100克体重的剂量可导致肌原纤维蛋白合成受到50%的抑制，其他两种组分的抑制率甚至更高。15分钟结束时，3.0毫克/100克体重的剂量可使肌浆蛋白和肌原纤维蛋白的合成受到96%的抑制。体内给予放线菌酮（0.3毫克/100克体重）可抑制细胞核中对α-鹅膏蕈碱有抗性的DNA依赖性RNA聚合酶活性。放线菌酮作用的抑制率与时间之间的关系符合一级动力学规律。这使得我们可以推测，参与rRNA转录的核蛋白中存在快速更新，其半衰期约为110分钟（通过图形确定）。有人提出了这种蛋白质参与系统性骨骼肌功能中rRNA合成适应性变化的观点。
